AND LABORATORY SAFETY IN THE ECE DEPARTMENT

Department: ___________________

Building: _____________________

Department Chairperson or Director: _________________________

Principal Investigator, Faculty Member, or Supervisor with Primary

Responsibility: _________________________

Designated Individuals with Specific/Supervisory Safety Responsibilities:

Person(s) designated by the principal investigator as subordinately responsible to

manage specific chemical hygiene plan functions eg. "responsible for chemical inventory

control" or "responsible for monthly eyewash testing").

Room(s) covered by this plan: __________________

Implementation date: __________________

Annual Review Dates: _________________

SITE SPECIFIC INFORMATION ON CHEMICAL RECEIVING,

STORING OR DISPENSING

(if applicable)

Give the location of your laboratory's chemical receiving, storage or

dispensing area. Describe any ordering policies or procedures for

hazardous chemicals. List any chemicals that require Principal Investigator

approval prior to purchase.

Receiving area(s): ____________________ .

Storage Area(s): _____________________ .

Special Dispensing Area(s): .

The following materials require approval by ______________________ prior

to ordering:

1. any regulated or known chemical carcinogen (section IX of the Laboratory Safety Guide

[LSG]);

2. any highly toxic material (in any physical state) and reproductive toxins (see Sec. XIII

and XIV);

3. any Drug Enforcement Administration controlled substance or precursor thereof.

NOTIFY GROUP INVENTORY MANAGER _____________________ OF ALL NEW CHEMICAL PURCHASES TO ENABLE UPDATING

CHEMICAL INVENTORY.

ORDERING AND ACQUISITION OF CHEMICALS

1. Group members should check for the availability of chemicals within the laboratory, or

other campus sources prior to ordering, especially when trial syntheses or special

operations/procedures are being evaluated. For high volume materials such as acetone

check with EH&S Material Handling Facility for recycled/redistilled solvents

2. A barcode should be applied to new containers received in the laboratory and the

computer based inventory should be updated to reflect receipt of the material.

3. Group members should contribute unused/uncontaminated chemicals to EH&S if it is

clear that further use of these particular chemicals within the group is very unlikely.

4. Order quantities of materials appropriate to intended uses. Order minimum practical

quantity of highly toxic and carcinogenic materials. Order time-sensitive materials with 6

month supply as an upper limit. These would include peroxide forming materials and

corrosive compressed gases. Examples of peroxide formers include; isopropyl and diethyl

ethers, 1,4 dioxane, tetrahydofuran, potassium metal. Examples of corrosive gases

include: silanes, hydrogen bromide, hydrogen fluoride, fluorine, hydrogen chloride,

ammonia.

STORAGE AND MAINTENANCE OF CHEMICALS

1) All hazardous material containers require examination at least annually. Chemicals

and their containers should be examined for signs of deterioration and label deterioration.

2) Chemicals should be divided and stored according to compatibility classes so that

accidental mixing between compatibility classes can reasonably be expected not to occur

(i.e. during earthquakes). Chemicals should be stored on shelves with lips or other

restraint. Shelf units should be secured to the wall. See Appendix I

3) Minimize quantities of flammable and combustible liquids stored in the open room.

Flammable and combustible liquids should be stored in Flammable Liquid Storage

Cabinets. If space is limited in flammable storage cabinets, avoid the practice of storing

nonflammable compatible liquids (eg. carbon tetrachloride) and reserve the space in the

flammable cabinet for flammable liquids.

4) Highly toxic/carcinogenic materials should be securely stored in a closed cabinet or

other isolated area where accidental contact or breakage will be prevented. Minimize

quantities on hand. Order smallest practical quantity available.

5) Water reactive materials should be stored away from potential contact with water, i.e.

do not store underneath the sink.

6) All Class 1A flammable liquids, such as diethyl ether, should be transferred in a fume

hood free of know ignition sources to 1 or 2 gallon or smaller safety cans for use in the

lab. 5 gallon drums must be stored in and approved flammable liquid storage cabinet or

storage room.

7) Every group member is responsible for proper labelling, storage and disposal of

materials and synthesized compounds in their work area(s).

8) Prior to leaving (permanently), a group member is responsible for clearing his/her work

area(s) along and proper disposition of all chemicals/materials to storage, waste, or to

another person's control (with their knowledge and agreement). All such materials must

be properly and fully labeled, a special emphasis shall be placed on laboratory

synthesized or non-commercial materials.

9) Cylinders should be separated according to compatibility class and stored chained or

strapped to a fixed surface. Two points of attachment should be used for securing

cylinders if possible. Cylinders should be turned off at the cylinder valve when not in use

and should be capped when stored.

10) Gas cylinders which are used to contain corrosive (or unstable) gases or liquids (such

as fluorine, nitrogen tetroxide, vinyl bromide, or hydrogen chloride) should always be

returned to the distributor within 6 months of the fill date indicated on the cylinder tag.

11) Group members should "date" time-sensitive materials upon receipt and upon

opening. Time-sensitive materials shall be marked with a tag to make them easily

identified. No materials should be used or tested after the manufacturers' "expiration

date" dates unless evidence of current stability has been obtained via direct testing prior

to the expiration date. NOTE: If material is very old (> 1yrs. past label expiration date) or

shows evidence of conversion to a hazardous status (ie. crystalline materials in/under cap

of ethers) then minimize handling and DO NOT OPEN OR ATTEMPT TO TEST - FAX or email EHS Waste disposal to request a special disposal for this item. Isolate from

possible inadvertent use until picked up. If tested (time frame for testing is based on the

nature of the material) the results must be indicated on container/tag with test date, test

results/method, and initials of (authorized) person conducting test. Examples (see also:

next page) of such materials would be peroxide forming chemicals (such as ethers),

materials which might polymerize or oxidize over time, materials which might become less

stable with dehydration (such as picric acid or perchloric acid).

Peroxide Detection Tests: The following tests will detect most peroxy compounds and all

hyperperoxides: NOTE: these tests should not be used for testing materials potentially

contaminated with inorganic peroxides (i.e. potassium)

Option 1. Add 1-3 ml of the liquid to be tested to an equal volume of acetic acid, add a

few drops of 5% potassium iodide (KI) solution and shake. The appearance of a yellow to

brown color indicates the presence of peroxides.

Option 2. Addition of 1 ml of a freshly prepared 10% KI and 10 ml of an organic

solution in a 25 ml glass cylinder should produce a yellow color if peroxides are present.

Option 3. Add 0.5 ml of the liquid to be tested to a mixture of 1 ml of 10% KI solution

and 0.5 ml of dilute hydrochloric acid to which a few drops of starch have been added

just before the test. The presence of a blue-black color within a minute indicates the

presence of peroxides.

Option 4. Peroxide test strips that turn an indicatative color in the presence of

peroxides. Care must be taken to follow manufacturer instructions for effective detection.

In general the strips must be air dried until the solvent evaporates and then exposed to

moisture for proper operation.

MSDS AND OTHER REFERENCE MATERIALS AVAILABLE IN

THE LABORATORY

Describe how and where MSDSs and other references are available in this

laboratory: (See the Bibliography for a list of recommended references).

_____________________________________________________________

Material Safety Data Sheets (MSDSs) are available in the following ways/locations:

_____________________________________________________________

Lab/Group: MSDSs received by any member of this research group are to be retained in

ROOM (Specific Location):

_____________________________________________________________

UCSD: Contact EHS (x-43660) ask for MSDS Coordinator

UCOP Systemwide: Electronic access (24 hrs/day, 7 days/week)

Telnet access: "telnet volga.ucop.edu" from computers on official campus LAN.

(access problems/questions call EHS (x-43660). See Appendix II.

MSDS are available via INFOPATH in the Environment, Health and Safety section of

campus information.

Over 11,000 MSDS are available via UCOP include all Fisher Scientific products, many

Kodak, plus many other distributers products.

Glove compatibility information is available in the UCSD Laboratory Safety Guide, in the

Fisher Catalog, in the Lab Safety Supply catalog. Glove suppliers may also be consulted

for specific applications.

For emergency information in cases of chemical contact call:

UC Medical Center/San Diego County Regional Poison Control Center at: 543-6000.

Information on reproductive hazards is available from the UCSD Teratogen Information service at 294-6084 and from EHS at 534-3660.

Building ________________________

Room(s )________________________

EMERGENCY RESPONSE INSTRUCTIONS

MEDICAL EMERGENCY: Immediate Procedures

*Remain calm.

*Initiate lifesaving measures if required.

*Call for Emergency Response 911.

*Do not move injured person unless there is danger of further harm.

*Keep injured person warm.

*UCSD Police or an ambulance should provide transport in most medical emergencies.

MAJOR INCIDENT: Immediate Procedures

*If it can be done safely, attend to injured or contaminated persons and remove them

from exposure.

*Alert people to evacuate the area.

*Call for Emergency Response:

Fire: 911

Chemical, radiation, biological spill: 534-3660

(Evenings & weekends): 534-4357 or 911

*Provide your name, location, and the phone number of person knowledgeable of

incident. That person should remain at the reported location until contacted by emergency

personnel.

*Close doors to affected areas

*If splash to the skin or eyes has occurred flush affected areas with copious quantities of

water for 15 minutes.

*Call the UCSD Poison Information Center 543-6000 for specific information

If the exposed individual is transported to a medical facility advise the Poison

Control Center of where the patient is going to allow continued assistance and to allow

Poison Control to alert the treating physicians of the nature of the exposure and the

appropriate medical response.

LABORATORY SPECIFIC PROCEDURES:

*Notify individuals in the area of the spill.

*If it can be done safely, attend to injured or contaminated persons and remove them from

exposure.

*Avoid breathing vapors of spilled material.

*Evacuate nonessential personnel. If spilled material is flammable, turn off ignition

and heat sources.

*Wear protective equipment.

*Confine spill to a small area, absorb and/or neutralize as appropriate. Collect residues,

place in container, and dispose of as chemical waste. NOTE: Do not neutralize spilled

liquid unless you are sure that the resulting reaction will not release hazardous gases,

products or cause explosion.

*Clean spill area with water or a buffer solution.

Supplies and Equipment: ___________________________________

1) Non-reactive absorbents such as oil-dry, vermiculite, or commercially available

absorbent pads should be available in the laboratory for liquid spills. NOTE: Paper

towels, may be used for incidental spills and for final wipe up, but caution should be

exercised because some chemicals, such as oxidizers, may cause ignition on contact or

in a delayed fashion through reaction.

2) Neutralizing agents including sodium carbonate or bicarbonate (for acid spills), and

citric acid (for basic spills) or commercial spill products will be stored in _____________

and reserved for spill use.

3) A plastic dust pan, whisk broom with heavy plastic bags will be stored in

_________________ for spill absorbent pickup.

4) Protective equipment including appropriate gloves, goggles and shoe covers should

be available.

5) A vacuum source, pipette, and a trap or a syringe can be used for mercury. Mercury

should be pushed into pools and then picked up. Mercury pick up pads may be used for

small droplets after the pickup of the majority of the material. Do not use sulfur or

other amalgamating materials for mercury cleanup. Disposal of non- elemental mercury has become extremely expensive and very difficult.

CALL EH&S FOR GUIDANCE AND ASSISTANCE FOR ALL SPILLS OF

MORE THAN AN INCIDENTAL NATURE.

ANY large, potentially dangerous spills should be reported to EH&S (days) and to x44357 or 911 (nights and weekends)immediately and to P.I. :___________________ or ______________________ and to the laboratory/departmental safety contact: ________________________.

ALL fires must be reported to the campus fire marshal (x43660). For any

uncontained fire: first pull fire alarm, dial 911 and report situation. Fires are

fought only when proper equipment/extinguishers are available and when other persons

are present to back you up and to help in reporting situation/progress to police.

If potential/actual exposure to hazardous materials has occurred contact Poison Control

Center (x43600).

Building:_____________ Room(s): ___________

SITE SPECIFIC ENGINEERING CONTROLS AND VENTILATION

INFORMATION

List protective engineering controls and systems available in the laboratory. Include

information on restrictions, special precautions or procedures, preventative maintenance

schedules and any other information relevant to safe operation in the laboratory.

Ventilation System: The ventilation system is a "one-pass" system. Air is supplied by

diffusers in the laboratory with exhaust provided by fume hoods. The laboratory is

maintained a negative pressure relative to the public areas such as hallways to prevent

spread of contaminants or accidental releases. There is no recirculation of exhausted air.

Fume Hoods: Fume hoods are to be used for containment of all work with volatile

hazardous compounds, hazardous dusts, gases and other materials that may constitute

an airborne exposure hazard unless other effective control measures are available.

1) Work shall be conducted at least 6 inches back from the front face of the hood. Storage in the hood shall be kept to a minimum practical level. The back baffles of the hood should

not be obstructed by excessive stored materials.

2) If the fume hood has not been tested within the last year or if there is a concern for

improper hood operation contact EH&S for a hood evaluation.

3) Fume hoods assigned to a primary user should be labeled with the name of the user and

a home and office phone number. If there are long term procedures performed, such as

overnight distillation, a short description of the procedure and materials should be posted

on the hood sash.

Environmental Rooms: Typically there is no ventilation to provide fresh air to

environmental rooms. Any use of toxic materials or flammable liquids in a warm or cold

room can result in a buildup of hazardous concentrations of airborne contaminants.

Flammable and toxic liquids or gases should not be used in environmental rooms.

Gas Cabinets: Gas cabinets shall be used for the use and storage of highly toxic gases.

Supply lines from the cabinets to the point of use shall be a continuous metal tube without

joints or connections. Tygon or other plastic tubing shall not be used for transport of highly

toxic or corrosive gas across laboratory space.

Glove Boxes:

Autoclaves:

See autoclave SOP

Building___________________________________

Room(s) ___________________________________

PERSONAL PROTECTIVE EQUIPMENT AVAILABLE

IN THE LABORATORY

List the personal protective equipment available to laboratory personnel.

See Chapter V for additional information.

Laboratory Attire: Laboratory workers should wear clothing appropriate to their

environment and laboratory operations. Typically closed toed shoes and clothing which

provides coverage for the extremities.

Eye Protection: Eye protection is required in all laboratory areas. The level and

selection of eye protection is based on the potential hazard presented from operations

conducted and the nature of the laboratory work in general. Safety glasses with side

shields which meet ANSI Z87.1 (all glasses which meet this standard have Z87.1

imprinted on the temple bar) is the minimum protection for all chemistry and biochemistry

laboratories.

1) Goggles will be used if a splash hazard exists with toxic or irritating chemicals. This

includes operation such as but not limited to: pouring liquids, such as organic solvents, at

or above eye level, pouring large quantities of liquids, using liquids under pressure.

2) A full faceshield with goggles will be used in instances such as when working with large quantities of liquids or gases that are toxic or corrosive, such as with phenol or

hydrochloric acid. A face shield with neck protection will be used if an explosion or

implosion hazard exists.

3) Glasses, a faceshield, or shielding designated by the manufacturer as providing

ultraviolet (UV) protection should be used when potential UV exposure exists (eg. using a

gelbox or using a UV lamp for chemical ionization).

Gloves: Many laboratory operations require skin and hand protection. Gloves will be

selected based on the chemical and physical hazards presented by an operation.

1) Disposable/single use latex gloves offer little or no protection from most solvents and can develop small holes that are often not readily apparent to the wearer. Single use latex

and should only be used if minimal consequences could result from skin contact.

2) Single use nitrile disposable gloves should be used for general laboratory operations.

The gloves should be changed frequently and whenever contamination has occurred.

3) Heavy gloves should be worn over the nitrile gloves when using large quantities of

chemicals or when contamination will be known to occur. The outer glove should be

selected based on chemical compatibility. Long gauntlet style gloves will be used when a potential exists for splash to the arm of significant quantities of corrosive or toxic materials.

Personal Protective Equipment- continued:

Other: A laboratory coat will be worn when working at the bench.

A neoprene or PVC apron shall be worn when working with large quantities of acids, with

corrosives under pressure, and with liquid phenol.

Building _____________________________

Room(s) _____________________________

PRIOR APPROVALS REQUIRED:

1) List prior approvals required for particular laboratory functions. The Principal Investigator or Laboratory Supervisor will determine which laboratory operations, if any will require prior approval.

2) Unattended or overnight operations.

3) Use of highly toxic gas (eg. nitric oxide, phosgene, hydrogen fluoride, arsine, phosphine) in any amount. Use of large quantities of toxic or corrosive gases.

4) Use of regulated carcinogens

Others: ________________________________

WORKING ALONE AND UNATTENDED OPERATIONS

Experiments known to be hazardous should not be conducted while alone in

the laboratory. Individuals performing low hazard tasks in off hours should make

arrangements with other individuals working in the building to cross check each other

periodically.

Laboratory operations that require continuous or overnight operation must have PI prior

approval. The process or experiment should be designed with provision to prevent

release of hazardous materials in the event of interruption of utilities such as electricity,

cooling water, or inert gas. Laboratory lights should be left on and a descriptive sign

posted identifying the nature of the experiment, the hazardous substances in use and

emergency contact information.

Building _________________________

Room(s) _________________________

STANDARD OPERATING PROCEDURES

READ AND REVIEW MSDS'S BEFORE USING ANY MATERIAL FOR THE FIRST TIME

1) Safety considerations will be included in initial experimental design. SOP's will be

developed and documented for hazardous chemicals when the chemical is used routinely

in the laboratory and when its use poses a potential hazard to laboratory personnel. All

highly toxic and toxic gases, unstable compounds that may explode, pyrophoric materials,

and highly toxic liquids and solids require development of an SOP even for a single event

due to hazards to personnel. Elements to be addressed when designing experiments or

procedures are: material hazards, availability of alternative safer materials, engineering

controls, personal protective equipment required, spill or release potential and possible

consequences, and other special considerations such as extreme reactivity.

2) SOP's may be developed by: process, such as distillation, peptide synthesis; each

hazardous chemical, such as cyanogen bromide; class of hazardous chemicals, such as

organic solvents or peroxidizable chemicals, or by any other reasonable approach that

addresses the health and safety concerns of the experiment.

3) SOP elements may be addressed in the laboratory notebook as part of the experiment or

process description or using the format provided on the SOP form. Elements 1 through 7

should be addressed for each process, class of chemicals, or individual chemical. For

"particularly hazardous substances" three additional elements, 8 through 10, must be

completed.

Element 1 Process or Experiment: State the process or type of process that involves the use of hazardous chemicals.

Element 2 Hazardous chemicals/Class of Hazardous Chemicals: State hazardous chemicals used or hazardous byproducts or reaction products produced.

Element 3 Personal Protective Equipment: State the personal protective equipment

selected and required and hygiene practices.

Element 4 Engineering/Ventilation Controls: Describe any specific engineering controls whose use is required to prevent employee exposures to hazards, such as fume hoods, interlocks on equipment, explosion shielding, ultraviolet light shielding,, and safety

features on equipment.

Element 5 Special Handling Procedures and Storage Requirements: Describe special storage requirements (eg. special containment devices, etc. Describe safe methods of transporting chemicals, such as double containment.

Element 6 Spill and Accident Procedures: Describe any special procedures for spills or releases (eg. neutralizing agents, use fluorescence to detect materials). Indicate how spills or accidental releases should be handled and by whom.

Element 7 Waste Disposal: Describe any special waste disposal procedures for these

chemicals/processes.

Element 8 Approval Required: Indicate if and when an approval from the PI or supervisor is required.

Element 9 Decontamination: Discuss any specific decontamination procedures for

equipment and glassware or areas.

Element 10 Designated Area: Indicate the designated area for work with this chemical or process. A designated area must be established for work with "particularly hazardous

substances" (See chapter XIV of the laboratory Safety Guide). The entire laboratory, fume

hood or a portion of the laboratory may be considered as a designated area.

APPENDIX I

GENERAL CHEMICAL COMPATIBILITY STORAGE GROUPS

1) Mineral (inorganic) Acids Examples: hydrochloric acid, sulfuric acid, phosphoric acid, boric acid, hydrobromic acid.

2) Oxidizers Examples: bromic acid, perchloric acid, chromic acid, nitric

acid, many perchlorates, permanganates, bromine, chlorine, fluorine, silver nitrate.

Note: Oxidizers should not be stored directly on wooden shelves or on paper shelf liners.

Spills may react with the organic portion of the shelf or paper and ignite spontaneously.

Perchloric acid presents special hazards and should be given special consideration to

assure effective isolation from oxidizable materials and dehydrating agents. Hypochlorite

solutions (i.e. bleach) are oxidizers however they will release chlorine gas on contact with

acids thus should be stored separately.

3) Bases/Caustics Examples: aqueous ammonia, ammonium hydroxide,

potassium hydroxide, sodium hydroxide.

4) Organic Solvents/Acids Examples: acetone, methanol, isopropyl ether,

methylene chloride, carbon tetrachloride, acetic acid, citric acid, benzene, tetrahydrofuran.

5) Highly Toxic/Carcinogenic Examples: sodium azide, acrolein, arsenic pentoxide,

pentachlorophenol, hydrazine, botulinum toxin, acrylamide, methyl isocyanate, phorbol

esters.

6) Pyrophoric Materials Examples: boron, diborane, dichloroborane, 2-

furaldehyde, diethyl aluminum chloride, lithium, white or yellow phosphorus, trimethyl

aluminum.

7) General "Dry" Examples: this would include many of the relatively Lab Chemicals innocuous or unreactive materials commonly found in laboratories.

8) Gases: Segregate also according to hazard class. Acutely toxic

and toxic gases should be stored in gas cabinets or fume hoods.

9) Water Reactives Examples: aluminum chloride-anhydrous, calcium

carbide, acetyl chloride, chlorosulfonic acid, phosphorous pentachloride, sodium,

potassium, calcium, aluminum tribromide, calcium oxide, acid anhydrides, metal hydrides.

10) Controlled Substances: Narcotics and other controlled substances should

be stored in a secure locked location such as a drawer or safe. See PPM Section 523-

2.2.1